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Echelon最新產(chǎn)品: EV?Purification?Kit?and?ExoTracker?kits 外泌體純化和標(biāo)記試劑盒
作者:Morey 發(fā)布時(shí)間:2020-11-19 點(diǎn)擊次數(shù):3006
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ExoClean and ExoTracker

K-4300a, K-4300b, K-4300c, K-4300d (20 tests)

Support: echelon@echelon-inc.com

Description: Simple, quick protocols for labeling (ExoTracker) and isolating (ExoClean) extracellular vesicles (EVs) from biological samples*

Echelon’s ExoClean – EV Purification Kit and ExoTracker kits為標(biāo)記和純化外顯體和細(xì)胞外囊泡(EV)提供了一個(gè)簡(jiǎn)單可靠的系統(tǒng)。1The ExoClean – EV Purification Kit (貨號(hào):K-4300a   )通過將沉淀試劑與微離心柱配對(duì),取代了與基于超離心的純化相關(guān)的時(shí)間和設(shè)備成本,此外可以去除與其他沉淀技術(shù)相關(guān)的有害污染物。https://echelon-inc.com/product/exoclean-ev-purification-kit/?mc_cid=a56b7d5013&mc_eid=c05a3cf4fc 

2The ExoTracker kits (貨號(hào):K-4300b -d) 使該系統(tǒng)的實(shí)用性更進(jìn)一步,可以用膜或RNA特異性標(biāo)簽標(biāo)記EV


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產(chǎn)品列表

Catalog #

Description

Quantity

ExoClean (K-4300a)

K-4301

EV Precipitation Reagent

30 mL

K-SPIN

Microcentrifuge Columns

20 columns

ExoTracker - Membrane (K-4300b)

K-4300a

ExoClean

1 kit

K-4302

EV Membrane Label

150 μg

ExoTracker - RNA (K-4300c)

K-4300a

ExoClean

1 kit

K-4303

EV RNA Label

20 μL

ExoTracker - Total (K-4300d)

K-4300a

ExoClean

1 kit

K-4302

EV Membrane Label

150 μg

K-4303

EV RNA Label

20 μL


操作流程:

1:Collect EV sample
2:Centrifuge at 2000g for 10 min to remove any debris or cells.
3:Transfer supernatant and precipitate cleared sample with-K4301 for 30 minutes at room temperature with mixing
4:Centrifuge precipitated sample at 1500g for 30 minutes at room temperature.
5:Discard the supernatant fraction and resuspend the precipitated pellet in 1x PBS.
6:Apply the resuspended pellet to the equilibrated K-SPINcolumn and incubate for 5 minutes at room temperature.
7:Centrifuge the column at 500g for 30 seconds at room temperature.
8:Collect and examine the eluted, purified EVs.

注:Exosomes and extracellular vesicles (EVs)-胞外體和胞外小泡

發(fā)表文獻(xiàn):

1. Konoshenko MY, Lekchnov EA, Vlassov AV, Laktionov PP. Isolation of Extracellular Vesicles: General Methodologies and Latest Trends. Biomed Res Int. 2018;2018:8545347.

2. Li P, Kaslan M, Lee SH, Yao J, Gao Z. Progress in Exosome Isolation Techniques. Theranostics. 2017;7(3):789-804.

3. Deregibus MC, Figliolini F, D’Antico S, Manzini PM, Pasquino C,?De Lena M, et al. Charge-based precipitation of extracellular?vesicles. Int J Mol Med. 2016;38(5):1359-66.

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